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Goat Anti-ORP9 / OSBPL9 Antibody (EB06158)

Related Products: EBP06158 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06158 Goat Anti-ORP9 / OSBPL9 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Fax. (888) 832-2613
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EB06158 1
EB06158 (2.5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: OSBPL9, ORP9, FLJ12492, FLJ14629, FLJ14801, FLJ32055, FLJ34384, MGC15035, oxysterol binding protein-like 9, OSBP-related protein 9, oxysterol-binding protein-related protein 9
Official Symbol: OSBPL9
Accession Number(s): NP_078862.4; NP_683702.1; NP_683704.2; NP_683705.1; NP_683706.3; NP_683707.3
Human GeneID(s): 114883
Important Comments: This antibody is expected to recognise all six human isoforms (NP_078862.4; NP_683702.1; NP_683704.2; NP_683705.1; NP_683706.3; NP_683707.3). Reported variants represent identical protein (NP_683702.1; NP_683703.1).

Immunogen

Peptide with sequence C-EPLLKRLGAAKH, from the C Terminus of the protein sequence according to NP_078862.4; NP_683702.1; NP_683704.2; NP_683705.1; NP_683706.3; NP_683707.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 150kDa and 19kDa in lysates of Human Brain, and Testis and 55kDa in lysates of Human Placenta and Testes after 0.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the different calculated sizes of the six reported isoforms (a: 62.5kDa for NP_683702.1 and NP_683703.1; b: 63.9kDa for NP_683704.1; c: 70.2kDa for NP_683705.1; d: 81.8kDa for NP_683706.1; e: 83.2 kDa for NP_078862.2; f: 84.3kDa for NP_683707.1). All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Lung shows staining of alveolar macrophages. . Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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