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Goat Anti-PAR6alpha / PARD6A Antibody (EB06228)

Related Products: EBP06228 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06228 Goat Anti-PAR6alpha / PARD6A Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06228 1
EB06228 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
EB06228 2
EB06228 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: partitioning-defective protein 6, partitioning defective-6 homolog alpha, par-6 partitioning defective 6 homolog alpha, Tax-interacting protein 40, PAR6C, PAR6, par-6 (partitioning defective 6, C.elegans) homolog alpha, Tax interaction protein 40, par-6 partitioning defective 6 homolog alpha (C.elegans), TIP-40, PAR-6A, TAX40, PAR-6, PAR6alpha, PARD6A
Official Symbol: PARD6A
Accession Number(s): NP_058644.1; NP_001032358.1
Human GeneID(s): 50855
Important Comments: This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1).

Immunogen

Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644.1; NP_001032358.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave no signal but low background in Human Brain and Pancreas extracts at up to 1µg/ml.
IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration, 10µg/ml. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocardial fibres in transverse section. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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