Change country
Tel: 888-320-GOAT (4628)
Fax: 888-841-9041
Home

Goat Anti-PAX8 (N Terminus) Antibody (EB05513)

Related Products: EBP05513 - Immunizing Peptide, EBP07037 - Immunizing Peptide, Goat Anti-PAX8 (internal) Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB05513 Goat Anti-PAX8 (N Terminus) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

Ordering Information for

Order direct from Everest

$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be processed and invoiced by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are placed with and invoiced by our US partner Abcore LLC.

 
EB05513 1
EB05513 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05513 2
EB05513 (3µg/ml) staining of paraffin embedded Human Thyroid Gland. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: paired box 8, paired domain gene 8, paired box gene 8, PAX8
Official Symbol: PAX8
Accession Number(s): NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1
Human GeneID(s): 7849
Non-Human GeneID(s): 18510 (mouse), 81819 (rat)
Important Comments: This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)

Immunogen

Peptide with sequence PHNSIRSGHGGLN-C, from the N Terminus of the protein sequence according to NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in Human Liver lysates (calculated MW of xxkDa according to NP_039245.1). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Thyroid Gland shows nuclear staining in epithelial cells around follicles. Recommended concentration, 1-2µg/ml. A customer found this antibody unsuccessful in immunohistochemistry on Mouse tissues.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Specific References

The goat polyclonal antibody used in this paper was manufactured by us:
Borgogni E, Sarchielli E, Sottili M, Santarlasci V, Cosmi L, Gelmini S, Lombardi A, Cantini G, Perigli G, Luconi M, Vannelli GB, Annunziato F, Adorini L, Serio M, Crescioli C.
Elocalcitol inhibits inflammatory responses in human thyroid cells and T cells.
Endocrinology. 2008 Jul;149(7):3626-34.
PMID: 18372324

The goat polyclonal antibody used in this paper was manufactured by us:
Crescioli C, Cosmi L, Borgogni E, Santarlasci V, Gelmini S, Sottili M, Sarchielli E, Mazzinghi B, Francalanci M, Pezzatini A, Perigli G, Vannelli GB, Annunziato F, Serio M.
Methimazole inhibits CXC chemokine ligand 10 secretion in human thyrocytes.
J Endocrinol. 2007 Oct;195(1):145-55.
PMID: 17911406

Product Reviews

Please login to review this product.

FAQs

 

Message from a distributor:

Please could you assist with this technical issue.  The customer saw positive staining in the membrane of catalog# EB05513 .  Please could you suggest a cause of this. Can you recommend a dilution and enzyme digestion?

Everest Reply:

Our EB05513 reacts with transcription factor PAX8 and it should be staining the nuclei. This is exactly what we have found in Human Thyroid Gland. It is possible however that this product does not work properly after different epitope retrieval methods. We obtained our IHC data after microwaved antigen retrieval with Tris/EDTA at pH9. A different pH value or a different heating type may result in different results.

I hope this will help your customer further. Please let me know when problems persist after adjustment of the epitope retrieval method.