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Goat Anti-PAX8 (N Terminus) Antibody (EB05513)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05513 | Goat Anti-PAX8 (N Terminus) Antibody | ELISA, IHC |  |
100µg specific antibody in 200µl | Human, Mouse, Rat | $300.00 |  |
|
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Sent via overnight courier arrives approximately next day to mainland USA. Order offlineNow shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (sales@everestbiotech.com), fax (888-841-9041), phone (888-320-GOAT) or mail. Please include the following information with your order:
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Target Protein
Principal Names: paired box 8, paired domain gene 8, paired box gene 8, PAX8Official Symbol: PAX8
Accession Number(s): NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1
Human GeneID(s): 7849
Non-Human GeneID(s): 18510 (mouse), 81819 (rat)
Important Comments: This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
Immunogen
Peptide with sequence PHNSIRSGHGGLN-C, from the N Terminus of the protein sequence according to NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Preliminary experiments gave a strong band at approx 40kDa in Human Liver lysates at 0.2ug/ml. Please note that currently we are unsure of the identity of this band given the existence of 5 isoforms of PAX8 between 450 aa and 287 aa. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Thyroid Gland shows nuclear staining in epithelial cells around follicles. Recommended concentration, 1-2µg/ml. A customer found this antibody unsuccessful in immunohistochemistry on Mouse tissues.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat
Specific References
The goat polyclonal antibody used in this paper was manufactured by us:
Borgogni E, Sarchielli E, Sottili M, Santarlasci V, Cosmi L, Gelmini S, Lombardi A, Cantini G, Perigli G, Luconi M, Vannelli GB, Annunziato F, Adorini L, Serio M, Crescioli C.
Elocalcitol inhibits inflammatory responses in human thyroid cells and T cells.
Endocrinology. 2008 Jul;149(7):3626-34.
PMID: 18372324
The goat polyclonal antibody used in this paper was manufactured by us:
Crescioli C, Cosmi L, Borgogni E, Santarlasci V, Gelmini S, Sottili M, Sarchielli E, Mazzinghi B, Francalanci M, Pezzatini A, Perigli G, Vannelli GB, Annunziato F, Serio M.
Methimazole inhibits CXC chemokine ligand 10 secretion in human thyrocytes.
J Endocrinol. 2007 Oct;195(1):145-55.
PMID: 17911406
Product Reviews
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Message from a distributor:
Please could you assist with this technical issue. The customer saw positive staining in the membrane of catalog# EB05513 . Please could you suggest a cause of this. Can you recommend a dilution and enzyme digestion?
Our EB05513 reacts with transcription factor PAX8 and it should be staining the nuclei. This is exactly what we have found in Human Thyroid Gland. It is possible however that this product does not work properly after different epitope retrieval methods. We obtained our IHC data after microwaved antigen retrieval with Tris/EDTA at pH9. A different pH value or a different heating type may result in different results.
I hope this will help your customer further. Please let me know when problems persist after adjustment of the epitope retrieval method.