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Goat Anti-PGC1A/PPARGC1A Antibody (EB07856)

Related Products: EBP07856 - Immunizing Peptide, EBP13106 - Immunizing Peptide, Goat Anti-PPARGC1A Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07856 Goat Anti-PGC1A/PPARGC1A Antibody Pep-ELISA, IF, IHC, FC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

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EB07856 1
EB07856 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07856 2
EB07856 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07856 3
EB07856 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB07856 4
EB07856 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07856 5
EB07856 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
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Target Protein

Principal Names: PGC1A, PPARGC1A, peroxisome proliferator-activated receptor gamma, coactivator 1 alpha, LEM6, PGC-1(alpha), PGC-1v, PGC1, PPARGC1, PPAR gamma coactivator variant form, PPAR gamma coactivator-1, ligand effect modulator-6, peroxisome proliferative activated receptor, gamma, coactivator 1, peroxisome proliferative activated receptor, gamma, coactivator 1, alpha
Official Symbol: PPARGC1A
Accession Number(s): NP_037393.1; NP_001317680.1; NP_001317681.1; NP_001317682.1; NP_001341756.1;
Human GeneID(s): 10891
Non-Human GeneID(s): 19017 (mouse), 83516 (rat)

Immunogen

Peptide with sequence C-DGLFDDSEDESDK, from the internal region of the protein sequence according to NP_037393.1; NP_001317680.1; NP_001317681.1; NP_001317682.1; NP_001341756.1;.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Preliminary testing showed a band at approx 90kDa in nuclear lysates of cell lines A431 and HEK293 after 0.3-1µg/ml antibody staining (calculated MW of 91.0kDa according to Human NP_037393.1). Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 6-8µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow