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Goat Anti-PIK3C2A Antibody (EB06291)

Code Name Applications Availability Size Species reactivity Price Grade  
EB06291 Goat Anti-PIK3C2A Antibody ELISA, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Dog $300.00 Elite

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EB06291 1
EB06291 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MGC142218, DKFZp686L193, C2-containing phosphatidylinositol kinase, phosphoinositide-3-kinase, class 2, alpha polypeptide, PI3-K-C2(ALPHA), PI3K-C2alpha, PI3-K-C2A, CPK, PIK3C2A
Official Symbol: PIK3C2A
Accession Number(s): NP_002636.2
Human GeneID(s): 5286
Non-Human GeneID(s): 18704 (mouse)

Immunogen

Peptide with sequence C-TVKWYQLTAATYL, from the C Terminus of the protein sequence according to NP_002636.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave no signal but low background in Human Testis and Molt-4 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Heart shows strong staining of the capillary network and patterned staining of the myocardial fibres in transverse section. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

Background Reference

Molz L, Chen YW, Hirano M, Williams LT.
Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain.
J Biol Chem. 1996 Jun 7;271(23):13892-9.
PMID: 8662856

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