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Goat Anti-Prolyl Endopeptidase / PREP Antibody (EB07251)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07251 | Goat Anti-Prolyl Endopeptidase / PREP Antibody | Pep-ELISA, IHC |  |
100µg specific antibody in 200µl | Human, Mouse, Rat, Dog, Pig, Cow | $315.00 |  |
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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: post-proline cleaving enzyme, dJ355L5.1 (prolyl endopeptidase), OTTHUMP00000040498, PEP, PE, MGC16060, HGNC:9358, prolyl endopeptidase, PREPOfficial Symbol: PREP
Accession Number(s): NP_002717.3
Human GeneID(s): 5550
Non-Human GeneID(s): 19072 (mouse), 83471 (rat)
Immunogen
Peptide with sequence C-QYIVGRSRKQSNP, from the internal region of the protein sequence according to NP_002717.3.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: Preliminary experiments gave an approx 45kDa band in Human Brain, Lymph Node, Spleen and Testis lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 80.7kDa according to NP_002717.3. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Kidney shows distinct staining patterns in the cytoplam of PCT. Recommended concentration, 3-6µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow