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Goat Anti-RACGAP1 / MgcRacGAP Antibody - Trial (EB05315-T)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05315-T | Goat Anti-RACGAP1 / MgcRacGAP Antibody - Trial | Pep-ELISA, WB, IHC |  |
20µg specific antibody in 40µl | Human, Dog, Cow | $105.00 |  |
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Ordering Information forOrder direct from Everest
$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be processed and invoiced by our US partner Abcore LLC. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: HsCYK-4, HsCYK-4, GTPase activating protein, MGCRACGAP, ID-GAP, Rac GTPase activating protein 1, MgcRacGAP, RACGAP1Official Symbol: RACGAP1
Accession Number(s): NP_037409.2; NP_001119576.1; NP_001119575.1
Human GeneID(s): 29127
Important Comments: Reported variants represent identical protein: NP_037409.2, NP_001119576.1 and NP_001119575.1.
Immunogen
Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.2; NP_001119576.1; NP_001119575.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 75kDa band observed in lysates of cell line K562 and Jurkat (calculated MW of 71kDa according to NP_037409). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration, 1µg/ml. Data obtained with a previous batch.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Dog, Cow
Specific References
The goat polyclonal antibody used in this paper was manufactured by us:
Seguin L, Liot C, Mzali R, Harada R, Siret A, Nepveu A, Bertoglio J.
CUX1 and E2F1 regulate coordinated expression of the mitotic complex genes Ect2, MgcRacGAP, and MKLP1 in S phase.
Mol Cell Biol. 2009 Jan;29(2):570-81.
PMID: 19015243
The goat polyclonal antibody used in this paper was manufactured by us:
Grewal S, Carver JG, Ridley AJ, Mardon HJ.
Implantation of the human embryo requires Rac1-dependent endometrial stromal cell migration.
Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16189-94.
PMID: 18838676
The goat polyclonal antibody used in this paper was manufactured by us:
Touré A, Mzali R, Liot C, Seguin L, Morin L, Crouin C, Chen-Yang I, Tsay YG, Dorseuil O, Gacon G, Bertoglio J.
Phosphoregulation of MgcRacGAP in mitosis involves Aurora B and Cdk1 protein kinases and the PP2A phosphatase.
FEBS Lett. 2008 Apr 9;582(8):1182-8.
PMID: 18201571
The goat polyclonal antibody used in this paper was manufactured by us:
Zhao WM, Seki A, Fang G.
Cep55, a microtubule-bundling protein, associateswith centralspindlin to control the midbody integrity and cell abscission during cytokinesis.
Mol Biol Cell. 2006 Sep;17(9):3881-96.
PMID: 16790497
The goat polyclonal antibody used in this paper was manufactured by us:
Nishimura Y, Yonemura S.
Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis.
J Cell Sci. 2006 Jan 1;119(Pt 1):104-14. Epub 2005 Dec 13.
PMID: 16352658