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Goat Anti-SAE1 / AOS1 Antibody (EB06097)

Related Products: EBP06097 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06097 Goat Anti-SAE1 / AOS1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Dog, Cow $315.00 Elite

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EB06097 1
EB06097 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ubiquitin-like 1-activating enzyme E1A, activator of SUMO1, UBLE1A, FLJ3091, SUMO1 activating enzyme subunit 1, ubiquitin-like protein SUMO-1 activating enzyme, SUMO-1 activating enzyme E1 N subunit, sentrin/SUMO-activating protein AOS1, SUMO-1 activating enzyme subunit 1, HSPC140, SUA1, AOS1, SAE1
Official Symbol: SAE1
Accession Number(s): NP_005491.1
Human GeneID(s): 10055

Immunogen

Peptide with sequence MKGNGIVECLGPK, from the C Terminus of the protein sequence according to NP_005491.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 40kDa band observed in Jurkat and 293 cell lysates (predicted MW of 40kDa according to NP_005491). Recommended for use at 1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Jones MC, Fusi L, Higham JH, Abdel-Hafiz H, Horwitz KB, Lam EW, Brosens JJ.
Regulation of the SUMO pathway sensitizes differentiating human endometrial stromal cells to progesterone.
Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16272-7.
PMID: 17053081

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