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Goat Anti-SLC10A2 / ASBT Antibody (EB08818)

Code Name Applications Availability Size Species reactivity Price Grade  
EB08818 Goat Anti-SLC10A2 / ASBT Antibody Pep-ELISA Yes 100µg specific antibody in 200µl Human $210.00 Aspiring

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Orders are placed with and invoiced by our US partner Abcore LLC.

 

Target Protein

Principal Names: ileal sodium-dependent bile acid transporter, ileal apical sodium-dependent bile acid transporter, OTTHUMP00000040691, NTCP2, ISBT, solute carrier family 10 (sodium/bile acid cotransporter family), member 2, ASBT, SLC10A2
Official Symbol: SLC10A2
Accession Number(s): NP_000443.1
Human GeneID(s): 6555

Immunogen

Peptide with sequence C-YKANGGFQPDEK, from the C Terminus of the protein sequence according to NP_000443.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx. 60kDa band in Human Kidney lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 37.7kDa according to NP_000443.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human

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