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Goat Anti-SMEK1 / KIAA2010 Antibody (EB07982)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07982 | Goat Anti-SMEK1 / KIAA2010 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB07982 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing SMEK1 (RC221040) and probed with EB07982 (mock transfection in first lane), tested by Origene.
Target Protein
Principal Names: SMEK homolog 1, suppressor of mek1 (Dictyostelium), KIAA2010, FLFL1, MSTP033, smk-1, smk1, SMEK homolog 1, suppressor of mek1Official Symbol: SMEK1
Accession Number(s): NP_115949.3
Human GeneID(s): 55671
Non-Human GeneID(s): 68734 (mouse), 314388 (rat)
Immunogen
Peptide with sequence C-EQQRERQDNPKLD, from the internal region of the protein sequence according to NP_115949.3.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: Approx 110kDa band observed in Human Pancreas lysates (calculated MW of 93.9kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog