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Goat Anti-SMUG1 Antibody (EB06321)

Related Products: EBP06321 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06321 Goat Anti-SMUG1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06321 1
EB06321 staining (0.5µg/ml) of Molt-4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MGC104370, single-strand-selective monofunctional uracil-DNA glycosylase 1, UNG3, HMUDG, FDG, single-strand selective monofunctional uracil DNA glycosylase, SMUG1
Official Symbol: SMUG1
Accession Number(s): NP_055126.1
Human GeneID(s): 23583

Immunogen

Peptide with sequence PQAFLLGSIHEPA-C, from the N Terminus of the protein sequence according to NP_055126.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35kDa band observed in Molt-4 lysates (predicted MW of 31kDa according to NP_055126. Recommended for use at 0.5-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Ma KW, Au SW, Waye MM.
Over-expression of SUMO-1 induces the up-regulation of heterogeneous nuclear ribonucleoprotein A2/B1 isoform B1 (hnRNP A2/B1 isoform B1) and uracil DNA glycosylase (UDG) in hepG2 cells.
Cell Biochem Funct. 2009 Jun;27(4):228-37.
PMID: 19384898

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