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Goat Anti-Spinesin / TMPRSS5 Antibody (EB05341)

Related Products: EBP05341 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05341 Goat Anti-Spinesin / TMPRSS5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $315.00 Elite

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EB05341 1
EB05341 staining (0.5µg/ml) of Human Brain (Cerebral Cortex) lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SPINESIN, MGC148044, MGC141886, transmembrane protease, serine 5 (spinesin), SPINESIN, TMPRSS5
Official Symbol: TMPRSS5
Accession Number(s): NP_110397.2
Human GeneID(s): 80975

Immunogen

Peptide with sequence C-LDWIHDTAQDSLL, from the C Terminus of the protein sequence according to NP_110397.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Experiments gave bands at approx 50kDa and 29kDa in Human Brain (Cerebral Cortex) lysates and in Mouse and Rat Brain lysates after 0.5µg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Yamaguchi et al, J Biol Chem. 2002 Mar 1;277(9):6806-12; PMID: 11741986). This protein has a calculated MW of 49.6kDa according to NP_110397.1. Recommended concentration: 0.5-1.5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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