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Goat Anti-TBL1X / TBL1XR1 Antibody (EB05313)

Related Products: EBP05313 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05313 Goat Anti-TBL1X / TBL1XR1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

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EB05313 1
EB05313 staining (4µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: transducin beta-like 1X, OTTHUMP00000022879, F-box-like/WD repeat-containing protein TBL1X, SMAP55, nuclear receptor co-repressor/HDAC3 complex subunit, TBL1-related protein 1, C21, DC42, FLJ12894, IRA1, TBLR1, transducin (beta)-like 1X-linked receptor 1, transducin (beta)-like 1 X-linked, transducin (beta)-like 1, transducin (beta)-like 1X-linked, TBL1, EBI, TBL1XR1, TBL1X
Official Symbol: TBL1X / TBL1XR1
Accession Number(s): NP_005638.1; NP_001132938.1; NP_001132939.1; NP_001132940.1; NP_078941.2
Human GeneID(s): 6907 , 79718
Important Comments: This antibody is also expected to recognise the human protein TBLR1 (NP_078941), which is very similar. Variants (NP_005638.1; NP_001132938.1) encode isoform a (TBL1X) and variants (NP_001132939.1; NP_001132940.1 ) encode isoform b (TBLX1).

Immunogen

Peptide with sequence SASDGSVCVLDLRK, from the C Terminus of the protein sequence according to NP_005638.1; NP_001132938.1; NP_001132939.1; NP_001132940.1; NP_078941.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 65-70kDa band seen in Human Brain lysate. Recommended for use at 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Hoberg JE, Yeung F, Mayo MW.
SMRT derepression by the IkappaB kinase alpha: a prerequisite to NF-kappaB transcription and survival.
Mol Cell. 2004 Oct 22;16(2):245-55.
PMID: 15494311

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