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Goat Anti-HNF1A Antibody (EB06701)

Code Name Applications Availability Size Tested species Price Grade  
EB06701 Goat Anti-HNF1A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06701 1
EB06701 (2µg/ml) staining of Rat Liver (A) and Mouse Liver (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06701 2
EB06701 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HNF1A, HNF1 homeobox A, HNF-1A, HNF1, TCF1, TCF-1, IDDM20, LFB1, MODY3, HNF-1-alpha, OTTHUMP00000198944, OTTHUMP00000240200, OTTHUMP00000240204, OTTHUMP00000240206, albumin proximal factor, hepatic nuclear factor 1, hepatocyte nuclear factor 1-alpha, interferon production regulator factor, liver-specific transcription factor LF-B1, transcription factor 1, hepatic
Official Symbol: HNF1A
Accession Number(s): NP_000536.5
Human GeneID(s): 6927
Non-Human GeneID(s): 21405 (mouse), 24817 (rat)

Immunogen

Peptide with sequence C-VIETFISTQMASSSQ, from the C Terminus of the protein sequence according to NP_000536.5.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 70kDa band observed in Rat Liver and Mouse Liver lysates (calculated MW of 67.4kDa according to NP_000536.5). Recommended concentration: 2-6µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Liver shows nuclear staining in hepatocytes. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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