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Goat Anti-TCP1 Antibody (EB06277)

Related Products: EBP06277 - Immunizing Peptide, Goat Anti-TCP1 Antibody - Trial
Code Name Applications Availability Size Species reactivity Price Grade  
EB06277 Goat Anti-TCP1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06277 1
EB06277 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06277 2
EB06277 (10µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: tailless complex polypeptide 1, T-complex protein 1, alpha subunit, T-complex protein 1, TCP-1-alpha, D6S230E, CCTa, CCT1, CCT-alpha, t-complex 1, TCP1
Official Symbol: TCP1
Accession Number(s): NP_110379.2; NP_001008897.1
Human GeneID(s): 6950
Important Comments: This antibody is expected to recognise both reported isoforms.

Immunogen

Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2; NP_001008897.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in Hela, Human Lung and Human Testis lysates (predicted MW of 60kDa according to NP_110379.2). Recommended for use at 0.5-2µg/ml.
IHC: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration, 1-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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