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Code Name Applications Availability Size Species reactivity Price Grade  
EB05914 Goat Anti-TRIM2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

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EB05914 1
EB05914 (0.5µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05914 2
EB05914 (6.3µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: tripartite motif protein TRIM2, RNF86, tripartite motif protein TRIM2, tripartite motif protein 2, KIAA0517, tripartite motif-containing 2, TRIM2
Official Symbol: TRIM2
Accession Number(s): NP_056086.2; NP_001123539.1
Human GeneID(s): 23321
Non-Human GeneID(s): 80890 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated.

Immunogen

Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2; NP_001123539.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 80+85kDa bands observed in Rat Brain lysates (calculated MW of 81.5kDa according to Human NP_001123539.1, 84.4 accorindg to Human NP_056086.2 and 83.3 according to Rat NP_001102022.1). Recommended concentration: 0.5-2µg/ml.. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Brain (Cortex) shows cytoplasm stainning in neuronal cell bodies. Recommended concentration, 6-12µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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