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Goat Anti-UNC5C Antibody (EB09218)

Code Name Applications Availability Size Species reactivity Price Grade  
EB09218 Goat Anti-UNC5C Antibody ELISA, IF Yes 100µg specific antibody in 200µl Human, Mouse, Dog $300.00 Elite

Ordering Information for

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Now shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (sales@everestbiotech.com), fax (888-841-9041), phone (888-320-GOAT) or mail. Please include the following information with your order:

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OR

Order from our local distributor

Axxora, LLC

6181 Cornerstone Court East Suite 103,
San Diego
CA 92121-4727
USA

Tel: 858-550-8830
Toll Free Tel: 1-800-550-3033
Fax: 858-550-8825
Toll Free Fax: 1-800-550-8825
Email: axxora-usa@axxora.com
Website: www.axxora.com

EB09218 1
EB09218 (50ug/ml) staining of islets in Mouse Pancreas. Citrate antigen retrieval. Detected by immunofluorescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: unc5C, unc5 (C.elegans homolog) c, homolog of C. elegans transmembrane receptor Unc5, UNC5H3, unc-5 homolog C (C. elegans), UNC5C
Official Symbol: UNC5C
Accession Number(s): NP_003719.2
Human GeneID(s): 8633
Non-Human GeneID(s): 22253 (mouse)

Immunogen

Peptide with sequence NCTVSEEPTGID, from the internal region of the protein sequence according to NP_003719.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in Human and Mouse Brain lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Immunofluorescence: Staining of paraffin embedded endocrine islets (red) in adult Mouse Pancreas. Nuclear staining in blue and ductal cell staining in green. Data obtained from anonymus customer.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Dog

Background Reference

Shin SK, Nagasaka T, Jung BH, Matsubara N, Kim WH, Carethers JM, Boland CR, Goel A.
Epigenetic and genetic alterations in Netrin-1 receptors UNC5C and DCC in human colon cancer.
Gastroenterology 2007 Dec 133 (6): 1849-57.
PMID: 18054557

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