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Goat Anti-USP20 / VDU2 Antibody (EB05997)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05997 Goat Anti-USP20 / VDU2 Antibody ELISA, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Pig, Cow $300.00 Elite

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EB05997 1
EB05997 (3µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: LSFR3A, ubiquitin specific peptidase 20, pVHL-interacting deubiquitinating enzyme 2, KIAA1003, ubiquitin specific protease 20, VDU2, USP20
Official Symbol: USP20
Accession Number(s): NP_006667.3
Human GeneID(s): 10868
Non-Human GeneID(s): 74270 (mouse)
Important Comments: All reported variants (NP_006667.3; NP_001103773.2; NP_001008563.2) encode the same protein.

Immunogen

Peptide with sequence C-HGEQKIEAETRAV, from the C Terminus of the protein sequence according to NP_006667.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave no signal but low background in Human Liver extracts at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Recommended concentration, 3 µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Background Reference

Curcio-Morelli C, Zavacki AM, Christofollete M, Gereben B, de Freitas BC, Harney JW, Li Z, Wu G, Bianco AC.
Deubiquitination of type 2 iodothyronine deiodinase by von Hippel-Lindau protein-interacting deubiquitinating enzymes regulates thyroid hormone activation.
J Clin Invest. 2003 Jul;112(2):189-96.
PMID: 12865408

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