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Goat Anti-Ymer / CCDC50 Antibody (EB06551)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06551 | Goat Anti-Ymer / CCDC50 Antibody | ELISA, IHC |  |
100µg specific antibody in 200µl | Human, Rat, Dog, Cow | $300.00 |  |
|
Ordering Information forOrder direct from Everest
$35.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Order offlineNow shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (sales@everestbiotech.com), fax (888-841-9041), phone (888-320-GOAT) or mail. Please include the following information with your order:
In stock orders received by 09.30 PST / 12.30 EST on Monday through Thursday are shipped the same day. OR
Order from our local distributorAxxora, LLC6181 Cornerstone Court East Suite 103, Tel: 858-550-8830 |
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Target Protein
Principal Names: chromosome 3 open reading frame 6, C3orf6, C3orf6, YMER, coiled-coil domain containing 50, Ymer proteinOfficial Symbol: CCDC50
Accession Number(s): NP_848018.1; NP_777568.1
Human GeneID(s): 152137
Important Comments: This antibody is expected to recognise both reported isoforms of human Ymer protein.
Immunogen
Peptide with sequence CFSKSESSHKGFHYK, from the internal region of the protein sequence according to NP_848018.1; NP_777568.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Preliminary experiments gave an approx 18kDa band in Human Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_848018.1 and 35.8kDa according to NP_777568.1. The 18kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Pancreas shows strong staining in cytoplasm in select acinar cells. Recommended concentration, 5-10µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Rat, Dog, Cow
Background Reference
Blagoev B, Ong SE, Kratchmarova I, Mann M.
Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics.
Nat Biotechnol. 2004 Sep;22(9):1139-45. Epub 2004 Aug 15.
PMID: 15314609