Goat Anti-Asporin / ASPN Antibody

$423.00

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SKU: EB06670
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
asporin proteoglycan|OS3|asporin|periodontal ligament associated protein 1|small leucine-rich protein 1C|asporin (LRR class 1)|FLJ20129|SLRR1C|PLAP1|ASPN
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.
Accession ID
NP_060150.4
Blocking Peptide
EBP06670
Immunogen
Peptide with sequence C-IHENKVKKIQKDT, from the internal region of the protein sequence according to NP_060150.4.
Peptide Sequence
C-IHENKVKKIQKDT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human, Mouse, Rat
Human Gene ID
54829
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 40kDa band observed in Human Tonsil and Uterus and in Mouse and Rat Skeletal Muscle lysates (calculated MW of 43.3kDa according to Human NP_060150.4, and 42.6kDa acciording to Mouse NP_001165952.1 and Rat NP_001014030.1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Cerebellum lysate.
Application Type
Pep-ELISA, WB, IF