Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names OTTHUMP00000063546\|BPG-dependent PGAM 2\|PGAM-M\|OTTHUMP00000020190\|BPG-dependent PGAM 1\|bisphosphoglycerate mutase 4\|bisphosphoglycerate mutase 2\|bisphosphoglycerate mutase 1\|phosphoglycerate mutase processed protein\|phosphoglycerate mutase family 4\|phosphoglycerate mutase family 3\|dJ1000K24.1\|PGAM-B\|phosphoglycerate mutase family member 4\|PGAM3\|Phosphoglycerate mutase, muscle form\|PGAMM\|MGC88743\|phosphoglycerate mutase 2 (muscle)\|Phosphoglycerate mutase A, nonmuscle form\|OTTHUMP00000059414\|RP11-452K12.8\|PGAMA\|phosphoglycerate mutase 1 (brain)\|PGAM2\|PGAM1
Accession ID NP_002620.1; NP_000281.2; NP_001025062.1
Blocking Peptide EBP05028
Immunogen Peptide with sequence C-KAMEAVAAQGKAKK, from the C Terminus of the protein sequence according to NP_002620.1; NP_000281.2; NP_001025062.1.
Product Comments Please note this antibody is expected to recognize the products of 3 highly similar genes.
Peptide Sequence C-KAMEAVAAQGKAKK
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species Human, Mouse, Rat, Pig
Human Gene ID 5223, 5224
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:128000.
Western Blot Approx 28kDa band observed in Human, Mouse, Rat and Pig Liver lysates (calculated MW of 28.8kDa according to NP_002620.1; NP_000281.2 and of NP_001025062.1). The additional 30kDa band observed in the rodent lysates likely represent the acetylated form. Recommended concentration: 0.05-0.15µg/ml. Primary incubation was 1 hour.
Application Type Pep-ELISA, WB

Selected References

[{"pmid": 30377371, "intro": "This antibody (previous batch) has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]

Goat Anti-PGAM1 / PGAM2 / PGAM4 Antibody

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