Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names orf1|bHLHd8|bHLHd7|bHLHd6|bHLHd5|bHLHd4|MYC associated factor X|helix-loop-helix zipper protein|myc-associated factor X|MGC36767|MGC34679|MGC18164|MGC11225|MGC10775|MAX protein|MAX | |
Usage Summary Immunofluorescence: Expression of the protein seen in the cytoplasm and nuclei of A431 and U251 cells. Recommended concentration: 10µg/ml.
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. | |
Accession ID NP_002373.3; NP_660087.1 | |
Blocking Peptide EBP05058 | |
Immunogen Peptide with sequence C-EEPQSRKKLRMEAS, from the C Terminus of the protein sequence according to NP_002373.3; NP_660087.1. | |
Product Comments This antibody is expected to recognize both reported isoforms (NP_002373.3; NP_660087.1). | |
Peptide Sequence C-EEPQSRKKLRMEAS | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow | |
Reactive Species Human | |
Human Gene ID 4149 | |
Mouse Gene ID 17187 | |
Product Grade  | |
IHC Results Paraffin embedded Human Prostate. Recommended concentration: 10µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 22kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3kDa according to NP_002373.3). Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, IHC |
Goat Anti-MAX Antibody
$464.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |