Goat Anti-PRAM1 Antibody

$423.00

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SKU: EB05159
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
PRAM-1|PRAM-1 protein|PML-RARA target gene encoding an Adaptor Molecule-1|PML-RARA regulated adaptor molecule 1|MGC39864|PML-RAR
Accession ID
NP_115528.4
Blocking Peptide
EBP05159
Immunogen
Peptide with sequence DFCDPLENQPLPLGR, from the C Terminus of the protein sequence according to NP_115528.4.
Peptide Sequence
DFCDPLENQPLPLGR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Pig
Reactive Species
Human
Human Gene ID
84106
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 90-100kDa band seen in Jurkat cell lysates. Recommended for use at 1-3µg/ml. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97 kDa and this is what we observed as well.
Application Type
Pep-ELISA, WB