Goat Anti-BPOZ / ABTB1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05200
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
PP2259|Btb3|elongation factor 1A binding protein|ankyrin repeat and BTB (POZ) domain containing 1|MGC20585|EF1ABP|BPOZ|ABTB1
Accession ID
NP_115937.1; NP_742024.1
Blocking Peptide
EBP05200
Immunogen
Peptide with sequence RALEDLLVSIGLDC, from the C Terminus of the protein sequence according to NP_115937.1; NP_742024.1.
Product Comments
This antibody is expected to recognise all reported isoforms (NP_115937.1; NP_742024.1; NP_742025.1).
Peptide Sequence
RALEDLLVSIGLDC
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human
Human Gene ID
80325
Mouse Gene ID
80283
Product Grade
IHC Results
Paraffin embedded Human Heart and Brain (Cerebellum). Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 38kDa band observed in Human Heart lysates, and approx. 50kDa in preliminary testing of MCF7 cell lysate (calculated MW of 37.6kDa according to NP_115937.1 and 54kDa according to ; NP_742024.1). An additional band was also consistently observed at 65-70xkDa. and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC

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