Goat Anti-PAX3 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06343
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
paired domain gene HuP2|paired box homeotic gene 3|MGC134778|MGC120384|MGC120383|MGC120382|MGC120381|paired box 3|paired box homeotic gene 3 (Waardenburg syndrome 1)|paired domain gene 3|paired box gene 3 (Waardenburg syndrome 1)|HUP2|CDHS|WS1|PAX3
Accession ID
NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1
Blocking Peptide
EBP06343
Immunogen
Peptide with sequence TTLAGAVPRMM-C, from the N Terminus of the protein sequence according to NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1.
Product Comments
This antibody is expected to recognize all 8 reported isoforms (NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1).
Peptide Sequence
TTLAGAVPRMM-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Cow
Reactive Species
Human
Human Gene ID
5077
Mouse Gene ID
18505
Rat Gene ID
114502
Product Grade
IHC Results
In paraffin embedded Human Esophagus expression of PAX3 is detected in squamous epithelium. Recommended concentration: 4.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 45kDa band observed in lysates of Human Duodenum, Skin, and in lysates of cell lines A431 (calculated MW of 45.2kDa according to NP_852125.1 and of 44.8kDa according to NP_852126.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC

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