Goat Anti-PPP2R5D Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform|delta isoform of regulatory subunit B56, protein phosphatase 2A|B56D|protein phosphatase 2, regulatory subunit B\1, delta isoform|Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform|PP2A, B subunit, PR61 delta isoform|PP2A, B subunit, B56 delta isoform|PP2A, B subunit, R5 delta isoform|PP2A, B subunit, B\1 delta isoform|protein phosphatase 2, regulatory subunit B (B56), delta isoform|MGC8949|MGC2134|PPP2R5D

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

NP_006236.1; NP_851307.1; NP_851308.1; NP_001257405.1

EBP05232

Peptide with sequence C-KRAEEFLTASQEAL, from the C Terminus of the protein sequence according to NP_006236.1; NP_851307.1; NP_851308.1; NP_001257405.1.

This antibody is expected to recognise reported isoforms (NP_006236.1 (iso 1); NP_851307.1; (iso 2) NP_851308.1) (iso 3) and NP_001257405.1 (iso 4).

C-KRAEEFLTASQEAL

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow

Human, Mouse, Rat

5528

Antibody detection limit dilution 1:20000.

Approx 70kDa band observed in lysates of cell lines A431, HepG2 and Jurkat, and in Human Cerebellum lysates, approx. 65-70kDa in lysates of cell line NIH3T3, and approx.75kDa in Rat Brain lysates, (calculated MW of 70kDa according to Human NP_006236.1, 69.0kDa according to Mouse NP_033384.2 and 77.8kDa according to Rat XP_017452220.1). Recommended concentration: 0.03-0.3µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IF, FC