Goat Anti-CRKL Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05254
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OTTHUMP00000198539|v-crk avian sarcoma virus CT10 oncogene homolog-like|v-crk sarcoma virus CT10 oncogene homolog (avian)-like|CRKL
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.

Accession ID
NP_005198.1
Blocking Peptide
EBP05254
Immunogen
Peptide with sequence C-KIFDPQNPDENE, from the C Terminus of the protein sequence according to NP_005198.1.
Peptide Sequence
C-KIFDPQNPDENE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human, Mouse
Human Gene ID
1399
Product Grade
IHC Results
In paraffin embedded Human Thymus shows staining of cellular membranes in thymocytes. Recommended concentration: 2.5-3.8µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 37kDa band observed in lysates of cell lines K562, Daudi and NIH3T3 (calculated MW of 33.8kDa according to Human NP_005198.1 and Mouse NP_031790.2 ). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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