Goat Anti-FOXP3 / SCURFIN Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

scurfin|FOXP3delta7|MGC141963|MGC141961|immune dysregulation, polyendocrinopathy, enteropathy, X-linked|immunodeficiency, polyendocrinopathy, enteropathy, X-linked|JM2 protein|DIETER|XPID|PIDX|IPEX|AIID|JM2|forkhead box P3|SCURFIN|FOXP3

Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Immunocytochemistry: CD25-sorted (Treg) Human blood cells with nuclear speckled staining. Recommended concentration 2-4ug/ml for overnight staining.

NP_054728.2; NP_001107849.1

EBP05294

Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2; NP_001107849.1.

This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)

SQRPSRCSNPTPGP

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

50943

Paraffin embedded Human Spleen. Recommended concentration: 6-8µg/ml.

Antibody detection limit dilution 1:8000.

Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.

Pep-ELISA, WB, IF, FC, ICC, IHC