Goat Anti-NUMB Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05296
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
numb homolog|S171|numb homolog (Drosophila)|NUMB
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cell junctions/plasma membrane and cytoplasm of A431 and NIH3T3 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001005743.1; 001005744.1; NP_003735; NP_001005745.1
Blocking Peptide
EBP05296
Immunogen
Peptide with sequence C-PFSSDLQKTFEIEL, from the C Terminus of the protein sequence according to NP_001005743.1; 001005744.1; NP_003735; NP_001005745.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
Peptide Sequence
C-PFSSDLQKTFEIEL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Dog, Cow
Reactive Species
Human, Mouse
Human Gene ID
8650
Mouse Gene ID
18222
Product Grade
IHC Results
Paraffin embedded Human Prostate. Recommended concentration: 6-8µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Application Type
Pep-ELISA, IHC, IF, FC

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