Goat Anti-RACGAP1 / MgcRacGAP Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05315
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
HsCYK-4|HsCYK-4|GTPase activating protein|MGCRACGAP|ID-GAP|Rac GTPase activating protein 1|MgcRacGAP|RACGAP1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1
Blocking Peptide
EBP05315
Immunogen
Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_001119575.1, NP_001306932.1, NP_001306930.1, NP_001306933.1
Peptide Sequence
C-GRQGNFFASPMLK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Cow
Reactive Species
Human
Human Gene ID
29127
Product Grade
IHC Results
In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 75kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0kDa according to NP_037409.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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