Goat Anti-SCAP2 / PRAP Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

src-associated adaptor protein|src kinase-associated phosphoprotein of 55-related protein|src family associated phosphoprotein 2|Pyk2/RAFTK-associated protein|Fyn-associated phosphoprotein SKAP55 homologue|SKAP55R|SKAP-HOM|SCAP2|SAPS|RA70|MGC33304|MGC10411|src family associated phosphoprotein 2|PRAP|SCAP2|SKAP2

Immunofluorescence: Strong expression of the protein seen in K562 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

NP_003921.2; NP_001290397.1

EBP05356

Peptide with sequence C-GLVPKAYIMEMYDI, from the C Terminus of the protein sequence according to NP_003921.2; NP_001290397.1.

C-GLVPKAYIMEMYDI

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

8935

Antibody detection limit dilution 1:3000.

Approx. 26+37kDa bands observed in lysates of cell line K562, and approx. 28+38kDa in preliminary testing of U937 cell lysate (calculated MW of 21.6kDa according to NP_001290397.1 and 41.2kDa according to NP_003921.2). Both bands were successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature. An additional band of unknown identity was also consistently observed in Jurkat at 28kDa. This band was successfully blocked by incubation with the immunising peptide.

Pep-ELISA, WB, IF, FC