Goat Anti-MARK4L Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05383
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
putative protein product of Nbla00650|FLJ90097|MAP/microtubule affinity-regulating kinase like 1|MARK4 serine/threonine protein kinase|Nbla00650|KIAA1860|MARKL1|MAP/microtubule affinity-regulating kinase 4|MARK4
Usage Summary
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_113605.2
Blocking Peptide
EBP05383
Immunogen
Peptide with sequence IKRKPPPGCSDSPGV, from the C Terminus of the protein sequence according to NP_113605.2.
Product Comments
This antibody is expected to recognize the reported long isoform 1 (NP_113605.2) only.
Peptide Sequence
IKRKPPPGCSDSPGV
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human, Mouse
Human Gene ID
57787
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 70kDa band observed in Human Cerebellum and Mouse Brain lysates, and in lysates of cell line U251 (calculated MW of 75.3kDa according to Human NP_113605.2 and 75.4kDa according to Mouse NP_001355356.1). This band was succefully blocked by incubation with the immunising peptide. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, FC

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