Goat Anti-KIF4A Antibody

$464.00

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SKU: EB05480
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
kinesin family member 4|chromokinesin|KIF4-G1|KIF4|HSA271784|FLJ20631|FLJ14204|FLJ12655|FLJ12530|kinesin family member 4A|KIF4A
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_036442.3
Blocking Peptide
EBP05480
Immunogen
Peptide with sequence SFFSGCSPIEEEAH, from the C Terminus of the protein sequence according to NP_036442.3.
Peptide Sequence
SFFSGCSPIEEEAH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Pig, Cow
Reactive Species
Human, Mouse
Human Gene ID
24137
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 150kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 139.5kDa according to Mouse NP_032472.2). Recommended concentration:1-2µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Ovary lysate.
Application Type
Pep-ELISA, WB, IF, FC