Goat Anti-IRF5 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05585
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
SLEB10|interferon regulatory factor 5|IRF5
Accession ID
NP_116032.1; NP_001092097.2; NP_001092099.1; : NP_001229381.1
Blocking Peptide
EBP05585
Immunogen
Peptide with sequence C-QGPWPMHPAGMQ, from the C Terminus of the protein sequence according to NP_116032.1; NP_001092097.2; NP_001092099.1; : NP_001229381.1.
Product Comments
This antibody is expected to recognize reported isoforms (NP_116032.1; NP_001092097.2 ; NP_001092099.1; : NP_001229381.1
Peptide Sequence
C-QGPWPMHPAGMQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
3663
Product Grade
IHC Results
Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx. 55+60kDa bands observed in Human Spleen lysates, approx. 65kDa in lysates of cell line A549, and approx. 70kDa in lysates of cell lines Daudi (calculated MW of 57.8kDa according to NP_001092099.1). These molecular weights have been observed by other commercial sources, and were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC

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