Goat Anti-Dynactin Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

p150, Glued homolog|dynactin 1|OTTHUMP00000202491|HMN7B|dynactin 1 (p150, Glued (Drosophila) homolog)|150 kDa dynein-associated polypeptide|p150, Glued (Drosophila) homolog|p150-glued|DAP-150|DP-150|P135|dynactin 1 (p150, glued homolog, Drosophila)|DCTN1

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells and additionally the nucleus of U2OS cells.. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1; NP_001177765.1; NP_001177766.1

EBP05639

Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1; NP_001177765.1; NP_001177766.1.

This antibody is expected to recognise all reported isoforms.

C-QEQLHQLHSRLIS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse

Human

1639

13191

Paraffin embedded Human Brain (Cortex). Recommended concentration: 6-7µg/ml.

Antibody detection limit dilution 1:16000.

Approx. 150kDa band observed in HeLa and MCF7 cell lysates, and in preliminary testing of HEK293 and Mouse Brain lysate (calculated MW of 142kDa according to NP_004073.2). Recommended concentration 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IF, IHC, FC