Goat Anti-PI31 / PSMF1 (Isoform 1) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05845
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
proteasome inhibitor subunit 1|proteasome inhibitor hP131 subunit|RP4-545L17.1|proteasome (prosome, macropain) inhibitor subunit 1 (PI31)|PI31|PSMF1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry:A customer reported weak staining of A431 cells at 10ug/ml.

Accession ID
NP_006805.2
Blocking Peptide
EBP05845
Immunogen
Peptide with sequence C-DHLPPPGYDDMYL, from the C Terminus of the protein sequence according to NP_006805.2.
Product Comments
This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).
Peptide Sequence
C-DHLPPPGYDDMYL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Mouse, Rat
Human Gene ID
9491
Mouse Gene ID
228769
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 30kDa band observed in lysates of cell line HEK293,, approx 30-32kDa in lysates of cell lines K562, NIH3T3, Neuro2a and KNRK, and approx. 35kDa in lysates of cell line LNCaP (calculated MW of 29.8kDa according to Human NP_006805.2 and Rat NP_001094475.1, and 28.7kDa according to Mouse NP_997611.1). Recommended concentration: 0.3-1µg/ml Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF

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