Goat Anti-NEIL1 / NEH1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05859
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
nei endonuclease VIII-like 1|hFPG1|FPG1|endonuclease VIII|FLJ22402|NEI1|nei endonuclease VIII-like 1 (E. coli)|NEH1|NEIL1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U937 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_078884.2; NP_001243481.1; NP_001339449.1
Blocking Peptide
EBP05859
Immunogen
Peptide with sequence C-DIPSLEPEGTSAS, from the C Terminus of the protein sequence according to NP_078884.2; NP_001243481.1; NP_001339449.1.
Peptide Sequence
C-DIPSLEPEGTSAS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
79661
Product Grade
IHC Results
In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration: 5-10µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx. 60kDa band observed in lysates of cell lines Daudi and U937, and in nuclear cell lysates of HEK293 (calculated MW of 53.0kDa according to NP_001243481.1). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 2-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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