Goat Anti-BAF57 / SMARCE1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06054
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
SMARCE1|BAF57|SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1|mammalian chromatin remodeling complex BRG1-associated factor 57|BAF57|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
Accession ID
NP_003070.3
Blocking Peptide
EBP06054
Immunogen
Peptide with sequence C-PPTDPIPEDEKKE, from the C Terminus of the protein sequence according to NP_003070.3.
Peptide Sequence
C-PPTDPIPEDEKKE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
6605
Mouse Gene ID
57376
Product Grade
IHC Results
In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration, 3-5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 50kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6kDa according to NP_003070.3). A 48kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 µg/ml. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EB06054 (mock transfection in first lane), tested by Origene. B) EB06054 staining (0.2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Application Type
Pep-ELISA, WB, IHC

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