Goat Anti-POLL / POLkappa Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06206
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
POLL|BETA-N|POL-KAPPA|polymerase (DNA directed), lambda|polymerase (DNA-directed), lambda|DNA polymerase kappa (DNA polymerase beta-N)|RP11-529I10.1|BETAN|FLJ46002|POLKAPPA|DNA polymerase beta-2|DNA polymerase kappa (DNA polymerase beta-N)|DNA polymerase lamda2|DNA-directed DNA polymerase lambda|OTTHUMP00000020323|pol beta2|pol lambda
Accession ID
NP_037406.1
Blocking Peptide
EBP06206
Immunogen
Peptide with sequence C-LGLPYREPAERDW, from the C Terminus of the protein sequence according to NP_037406.1.
Product Comments
It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.
Peptide Sequence
C-LGLPYREPAERDW
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
27343
Mouse Gene ID
56626
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 60kDa band observed in cell lysates of A549 and approx 60-70kDa in cell lysates of HepG2 (calculated MW of 63.5kDa according to Human NP_037406.1). Recommended concentration: 0.5-1µg/ml.
Application Type
Pep-ELISA, WB

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