Goat Anti-4E-T / EIF4ENIF1 Antibody

$423.00

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SKU: EB06230
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OTTHUMP00000063276|FLJ26551|eIF4E-transporter|eukaryotic translation initiation factor 4E nuclear import factor 1|2610509L04Rik|FLJ21601|Clast4|4E-T|EIF4ENIF1
Accession ID
NP_062817.2; NP_001157974.1
Blocking Peptide
EBP06230
Immunogen
Peptide with sequence C-AKVISVDELEYRQ, from the C Terminus of the protein sequence according to NP_062817.2; NP_001157974.1.
Product Comments
This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
Peptide Sequence
C-AKVISVDELEYRQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
56478
Mouse Gene ID
74203
Product Grade
IHC Results
In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5µg/ml
Application Type
Pep-ELISA, WB, IHC