Goat Anti-TCP1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

tailless complex polypeptide 1|T-complex protein 1, alpha subunit|T-complex protein 1|TCP-1-alpha|D6S230E|CCTa|CCT1|CCT-alpha|t-complex 1|TCP1

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_110379.2; NP_001008897.1

EBP06277

Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2; NP_001008897.1.

This antibody is expected to recognise both reported isoforms.

C-SYEDAVHSGALND

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

6950

In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration: 1-2µg/ml.

Antibody detection limit dilution 1:32000.

Approx 60kDa band observed in lysates of cell line HEK293 and in Human Ovary lysates (calculated MW of 60.3kDa according to NP_110379.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, IF, FC