Goat Anti-RGS14 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06368
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
regulation of G protein signaling 14|regulator of G-protein signalling 14|RGS14
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.

Accession ID
NP_006471.2; NP_001353546.1
Blocking Peptide
EBP06368
Immunogen
Peptide with sequence C-IGGSLNSTTDSAL, from the C Terminus of the protein sequence according to NP_006471.2; NP_001353546.1.
Peptide Sequence
C-IGGSLNSTTDSAL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
10636
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary testing showed a band at approx. 70kDa in Daudi and THP-1 cell lysate after 1-3ug/ml antibody staining. (calculated MW of 61.4kDa according to NP_006471.2). This molecular weight is observed by other sources. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC

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