Goat Anti-58KGolgi protein(Internal)/FTCD Antibody

$464.00

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SKU: EB06443
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
LC1 autoantigen|formimidoyltransferase cyclodeaminase|formiminotransferase cyclodeaminase|LCHC1|FTCD
Usage Summary
Immunofluorescence: Expression of the protein seen in the membranes and cytoplasm of Caco-2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: A customer reported weak staining on Caco-2 cells at a concentration of 10ug/ml.
Accession ID
NP_006648.1; NP_996848.1; NP_001307341.1
Blocking Peptide
EBP06443
Immunogen
Peptide with sequence CLREQGRGKDQPGRL, from the internal region of the protein sequence according to NP_006648.1; NP_996848.1; NP_001307341.1.
Product Comments
Variants (NP_006648.1; NP_996848.1) encode the same protein.
Peptide Sequence
CLREQGRGKDQPGRL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Pig, Cow
Reactive Species
Human, Mouse, Rat
Human Gene ID
10841
Product Grade
IHC Results
Paraffin embedded Human Liver. Recommended concentration: 5-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 55-58kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 58.9kDa according to Human NP_006648.1, Mouse NP_543121.1 and Rat NP_446019.1). Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC