Goat Anti-EndoPDI / TXNDC5 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

thioredoxin domain containing 5|protein disulfide isomerase family A, member 15|endothelial protein disulphide isomerase|PDIA15|Hcc-2|thioredoxin domain containing 5 (endoplasmic reticulum)|TXNDC5|endothelial protein disulphide isomerase|thioredoxin related protein|FLJ90810|FLJ21353|MGC3178|UNQ364|ERP46|thioredoxin domain containing 5|EndoPDI

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum of U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_110437.2; NP_001139021.1

EBP06471

Peptide with sequence C-SLHRFVLSQAKDEL, from the C Terminus of the protein sequence according to NP_110437.2; NP_001139021.1.

This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437.2; NP_001139021.1.

C-SLHRFVLSQAKDEL

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

81567

Paraffin embedded Human Small Intestine. Recommended concentration: 3.75µg/ml.

Antibody detection limit dilution 1:64000.

Approx 50kDa band observed in lysates of cell lines HEK293, A549, HeLa and HepG2 (calculated MW of 47.6kDa according to NP_110437.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-0.1µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, IF, FC