Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names OTTHUMP00000069747|FLJ44618|FLJ42622|testilin|EH-domain containing 1|HPAST1|H-PAST|PAST1|PAST|EHD1 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_001269373.1; NP_001269374.1 | |
Blocking Peptide EBP06492 | |
Immunogen Peptide with sequence FSWVSKDARRKKEPC, from the N Terminus of the protein sequence according to NP_001269373.1; NP_001269374.1. | |
Peptide Sequence FSWVSKDARRKKEPC | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 10938 | |
Product Grade  | |
IHC Results Paraffin embedded Human Thymus and Brain (Cortex). Recommended concentration: 3.75µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 60kDa band observed in lysates of cell line NIH3T3 (calculated MW of 60.6kDa according to Mouse NP_0342491) Preliminary testing also showed a band at approx 60kDa in Human Lymph node and Rat Kidney lysate and in HeLa cell lysate. Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, IHC, FC |
Goat Anti-EHD1 Antibody
$464.00
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