Goat Anti-Fhl1/ Slim1 (mouse) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06507
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
SLIM|LIM protein SLIMMER|SLIM-1|FLH1A|FHL-1|Isl-1 and Mec-3 domains 1|four-and-a-half Lin11|XMPMA|SLIMMER|MGC111107|KYOT|FHL1B|skeletal muscle LIM-protein1|Four-and-a-half LIM domains 1|four and a half LIM domains 1|FHL1A|RP11-535K18.1|KYO-T|SLIM1|FHL1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001070830.1; NP_001274729.1
Blocking Peptide
EBP06507
Immunogen
Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001070830.1; NP_001274729.1.
Product Comments
This antibody is expected to recognize Mouse NP_001070830.1 iso 2 , NP_001274729.1 iso 3 and Rat NP_001029098.1 iso 1, NP_001258128.1 iso 3.
Peptide Sequence
CNKRFVFHNEQVY
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Reactive Species
Human, Mouse, Rat
Mouse Gene ID
14199
Rat Gene ID
25177
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 32kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates and in Rat Lung and Testis lysates (calculated MW of 31.9kDa according to Mouse NP_001274729.1 and Rat NP_001258128.1). Recommended concentration: 0.01-0.5µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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