Goat Anti-LXR alpha / LXR beta Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06522
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
oxysterols receptor LXR-beta|nuclear orphan receptor LXR-beta|liver X receptor-beta|iver X receptor beta|LX receptor beta|LXRB|liver X receptor-alpha|liver X receptor alpha|OTTHUMP00000198038|ubiquitously-expressed nuclear receptor|nuclear receptor subfamily 1, group H, member 2|RIP15|NER-I|LXR-b|UNR|NER|ubiquitously-expressed nuclear receptor / NR1H2|nuclear receptor subfamily 1, group H, member 2|RIP15|NER-I|LXR-b|UNR|NER|NR1H2|liver X receptor, alpha|nuclear receptor subfamily 1, group H, member 3|NR1H3|RLD-1|LXR-a|LXRA
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_005684.2; NP_009052.4;NP_001123573.1; NP_001238863.1; NP_001350524.1
Blocking Peptide
EBP06522
Immunogen
Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2; NP_009052.4;NP_001123573.1; NP_001238863.1; NP_001350524.1.
Product Comments
This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
Peptide Sequence
CRLQDKKLPPLLSEI
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
10062, 7376
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 55kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on NIH3T3 and Rat for this particular batch.
Application Type
Pep-ELISA, WB, IF, FC

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