Goat Anti-Ymer / CCDC50 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

Ymer protein|coiled-coil domain containing 50|C3orf6, YMER|C3orf6|chromosome 3 open reading frame 6

NP_848018.1; NP_777568.1

EBP06551

Peptide with sequence CFSKSESSHKGFHYK, from the internal region of the protein sequence according to NP_848018.1; NP_777568.1.

This antibody is expected to recognise both reported isoforms of human Ymer protein.

CFSKSESSHKGFHYK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Rat, Dog, Cow

Human

152137

In paraffin embedded Human Pancreas shows strong staining in cytoplasm in select acinar cells. Recommended concentration, 5-10µg/ml.

Antibody detection limit dilution 1:32000.

Preliminary experiments gave an approx 18kDa band in Human Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_848018.1 and 35.8kDa according to NP_777568.1. The 18kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC