Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names triose-phosphate isomerase|TPID|TIM|HEL-S-49|epididymis secretory protein Li 49|MGC88108|TPI|TPI1|triosephosphate isomerase 1 | |
Usage Summary Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_000356.1; NP_001152759.1; NP_001244955.1 | |
Blocking Peptide EBP06643 | |
Immunogen Peptide with sequence C-LKPEFVDIINAKQ, from the C Terminus of the protein sequence according to NP_000356.1; NP_001152759.1; NP_001244955.1. | |
Product Comments This antibody is expected to recognise reported isoforms 1, 2 and 3 (NP_000356.1; NP_001152759.1, NP_001244955.1. | |
Peptide Sequence C-LKPEFVDIINAKQ | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow | |
Reactive Species Human, Mouse, Rat, Dog | |
Human Gene ID 7167 | |
Mouse Gene ID 21991 | |
Rat Gene ID 24849 | |
Product Grade  | |
IHC Results In paraffin embedded Human Liver shows staining of the sinusoids. Recommended concentration: 2-4µg/ml. Paraffin embedded Human Pancreas. Recommended concentration: 5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx 26kDa band observed in lysates of cell lines HEK293, HeLa, HepG2, Jurkat, Raw264.7. KNRK and MDCK (calculated MW of 26.7kDa according to Human NP_000356.1). Recommended concentration: 0.001-0.01µg/ml. Primary incubation 1 hour at room temperature. This antibody has been successfully used in WB on Mouse in PMID: 18544163. This antibody has been successfully used in WB on Human in PMID: 17326818. | |
Application Type Pep-ELISA, WB, IHC, FC |
Goat Anti-Triosephosphate isomerase Antibody
$464.00
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Selected References [{"pmid": 17326818, "intro": "This antibody has been successfully used in Western blot on Human:", "title": "Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis--a proteomic study.", "author": "Vorum H, Østergaard M, Rice GE, Honoré B, Bek T.", "journal": "Proteome Sci. 2007 Feb 27;5:5."}, {"pmid": 18544163, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53.", "author": "Honoré B, Buus S, Claësson MH.", "journal": "Proteome Sci. 2008 Jun 10;6:18."}] |