Goat Anti-RBP1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06671
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
CRBPI|retinol-binding protein 1, cellular|retinol binding protein 1, cellular|CRABP-I|CRBP1|RBPC|CRBP|RBP1
Usage Summary
Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002890.2
Blocking Peptide
EBP06671
Immunogen
Peptide with sequence VEGVVCKQVFKKVQ, from the C Terminus of the protein sequence according to NP_002890.2.
Peptide Sequence
VEGVVCKQVFKKVQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Cow
Reactive Species
Human, Mouse
Human Gene ID
5947
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx 16kDa band observed in NIH3T3 and approx 17kDa in U251 cell lysates. This molecular weight is routinely observed by other sources. (calculated MW of 22.3kDa according to Human NP_002890.2 and 15.8kDa according to Mouse NP_035384.1). Recommended concentration: 0.5-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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